Evaluation of High‐resolution Melting Curve Analysis as a New Tool for Root‐knot Nematode Diagnostics |
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| Authors: | Martijn H M Holterman Markus Oggenfuss Sebastian Kiewnick |
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| Institution: | Authors’ address: Agroscope Changins‐Waedenswil, Research Station ACW, Schloss, P.O. Box 185, 8820 Waedenswil, Switzerland (correspondence to S. Kiewnick. E‐mail: sebastian.kiewnick@acw.admin.ch) |
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| Abstract: | In the detection of plant pests, speed and accuracy are vital. High‐resolution melting curve (HRMC) analysis was therefore evaluated as a new tool for the identification of root‐knot nematodes (Meloidogyne spp.). On the basis of the second intergenic spacer (IGS2) region of the ribosomal DNA cistron, Meloidogyne chitwoodi, M. fallax and M. hapla were successfully distinguished from each other and the group of the three tropical species, M. incognita, M. arenaria and M. javanica. Conversely, it was shown that the IGS2 region is not suitable for the tropical species M. enterolobii (senior synonym of M. mayaguensis) as the amplification of multiple fragments of different lengths prevented a reliable HRMC analysis. However, the obtained results provide a proof of principle that HRMC analysis can be a suitable single‐tube assay for fast and accurate root‐knot nematode identification. |
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| Keywords: | high‐resolution melting curve analysis IGS2 molecular diagnostics Meloidogyne spp ribosomal DNA root‐knot nematode species identification |
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