| 结核分枝杆菌Ag85A DNA的克隆与原核表达 |
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| 引用本文： | 姜燕,Azuma Kalu,吕昌龙,单风平. 结核分枝杆菌Ag85A DNA的克隆与原核表达[J]. 微生物学杂志, 2007, 27(3): 16-20 |
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| 作者姓名： | 姜燕 Azuma Kalu 吕昌龙 单风平 |
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| 作者单位： | 中国医科大学,基础医学院免疫学研究室,辽宁,沈阳,110001 |
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| 摘 要： | 抗原85(Ag85)复合体是BCG合成分泌的可刺激机体产生细胞免疫和体液免疫应答。Ag85A是抗原85复合体组成成分之一,可显著刺激细胞免疫功能增强。研究构建了高效表达的pTrcHisB-Ag85A基因重组质粒,并将其转化至大肠埃希菌TOP10中进行重组蛋白的表达,筛选转化物,SDS-PAGE进行蛋白表达的分子量检测,并通过Western Blotting方法,应用抗Ag85A的单克隆抗体进一步确认重组Ag85A蛋白的表达,建立了Ag85A的原核表达体系,为进一步制备出有效的重组Ag85A疫苗奠定基础。
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| 关 键 词： | 重组DNA 结核分枝杆菌 表达载体 |
| 文章编号： | 1005-7021(2007)03-0016-05 |
| 修稿时间： | 2007-01-20 |
Clone and Prokaryotic Expression of Mycobacterium tuberculosis Ag85A DNA |
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| JIANG Yan,Azuma Kalu,LV Chang-long,SHAN Feng-ping. Clone and Prokaryotic Expression of Mycobacterium tuberculosis Ag85A DNA[J]. Journal of Microbiology, 2007, 27(3): 16-20 |
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| Authors: | JIANG Yan Azuma Kalu LV Chang-long SHAN Feng-ping |
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| Abstract: | Antigen 85(Ag85) complex is synthetic secretion BCG that can stimulate organism to produce cell immune and body fluid immune response.It can remarkably stimulate cell immune to strengthen.High-effective gene recombinant plasmid pTrcHisB-Ag85A was constructed in this study,and transferred into E.coli TOP10 to carry out recombinant protein expression,then screen for transformants,test molecularly for protein expression by SDS-PAGE,and further test for confirming the recombinant protein expression of Ag85A with its monoclonal antibody through Western Blotting,thus to construct prokaryotic expression system of Ag85A to lay a foundation for further preparation effective recombinant Ag85A vaccine. |
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| Keywords: | Ag85A |
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