三叶青叶表型多样性与地上部分总黄酮含量相关性

为揭示不同种源三叶青（Tetrastigma hemsleyanum Diels & Gilg）叶表型多样性变化及其与地上部分总黄酮含量的相关性，以49个三叶青种质资源（简称“资源”）为研究对象，对各资源叶表型的12个长度性状及7个比例性状进行比较，并采用相关性分析、因子分析和聚类分析方法对叶表型性状及其与种源地位置和地上部分总黄酮含量关系进行统计分析。结果表明：各资源叶长度性状存在较大差异，其中，小叶柄和侧生小叶叶片相关性状在三叶青资源鉴别过程中具有较高的参考价值；部分三叶青叶长度性状与比例性状间存在相关性，整体表现为叶柄增长，小叶叶片则更宽阔，而小叶叶片增长，叶片则朝狭长方向发展；中叶长和侧叶柄长等部分叶形性状与种源地纬度、经度或海拔存在显著（P<0.05）或极显著（P<0.01）相关性，可作为种源地地理位置初步判断依据；基于3个叶形公因子的聚类分析结果显示，49个三叶青资源叶形可划分为5个类型，各省（直辖市）三叶青叶类型数量呈以江西为中心，向周围辐射递减的趋势；三叶青地上部分总黄酮含量与种源地地理位置无显著相关性，但与叶片性状公因子（公因子1）呈极显著负相关（P<0.01），与叶柄长度性状公因子（公因子2）呈极显著正相关（P<0.01）。本研究完善了三叶青形态学资料，为三叶青优良品种选育提供了形态学依据。

The Correlation Between Leaf Phenotype Diversity and Total Flavonoids Content of Overground Part of Tetrastigma hemsleyanum Diels & Gilg

To reveal the variation of leaf phenotypic diversity of Tetrastigma hemsleyanum Diels & Gilg from different origin and its correlation with the total flavonoids content of overground part. In this paper， 49 germplasm resources（abbreviation resources） of T. hemsleyanum were selected as materials， and 12 characters of length and 7 proportion characters of leaf phenotype were compared. The phenotypic characters of leaf and their relationship with origin location and total flavonoids content were statistically analyzed by correlation analysis， factor analysis and cluster analysis. The results showed that there were significant differences in leaf length characters among resources， and the leaf characters of petiolule and lateral leaflet were of high reference value in the identification of T. hemsleyanum resources； There was correlation between leaf length characters and proportion characters， which showed that leaflet was wider when petiole grew， and leaflet was longer and narrower while leaflet elongated； Some leaf shape characters， such as central leaflet length and lateral petiolule length， were significantly（P<0.05） or extremely significantly（P<0.01） correlated with latitude， longitude or altitude of origin location， which indicated that these characters could be used as preliminary basis for geographical location estimation of origin； The cluster analysis based on 3 common factors of leaf shape showed that the leaf shape of 49 resources of T. hemsleyanum could be divided into five types， and the number of leaf type in each origin（municipalities） showed a radially decreasing trend from Jiangxi to the surrounding area； There was no significant correlation between the total flavonoids content of the overground parts and the geographical location of origin， but total flavonoids content was significantly negatively correlated（P<0.01） with the common factor of leaf blade characters（common factor 1）， and significantly positively correlated（P<0.01） with the common factor of petiole length characters（common factor 2）. The results improved the morphological data of T. hemsleyanum and provided the morphological basis for the breeding of excellent varieties of T. hemsleyanum.