Tryptophan decarboxylase from transformed roots of Catharanthus roseus |
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Authors: | Islas-Flores Ignacio Moreno-Valenzuela Oscar Minero-García Yereni Loyola-Vargas Victor M Miranda-Ham María de Lourdes |
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Affiliation: | (1) Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigacíon Científica de Yucatán, Calle 43 # 130, Chuburná de Hidalgo, 97200 Mérida, Yucatán, México |
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Abstract: | Tryptophan decarboxylase (TDC, EC 4.1.1.28) from Catharanthus roseus hairy roots was purified 80-fold. Antibodies against TDC were obtained and they recognized only one protein of 55 kDa in crude extracts from hairy root cultures. Elicitation of transformed root cultures with macerozyme yielded a marked increase in TDC activity, which was accompanied by a similar increase in the amount of immunoreactive TDC protein. These results suggest that the alkaloid accumulation, produced by elicitation, requires the synthesis of new TDC polypeptide in C. roseus root cultures and establishes important differences in the regulatory control of this enzyme in root cultures compared to developing seedlings, where the posttranslational regulation apparently plays a major role. |
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Keywords: | Tryptophan decarboxylase transformed roots Catharanthus roseus elicitors secondary metabolism |
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