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Long-term resistance to tomato spotted wilt virus in transgenic tobacco cultivars expressing the viral nucleoprotein gene: greenhouse and field tests |
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| Authors: | Stoeva Pravda Yankulova Margarita Nikolaeva Velichka Bachvarova Rositza Ivanova Lidia Maiss Edgar Adam Guenter Vulkov Vladimir Guelemerov Spas Atanassov Atanas |
| Affiliation: | (1) The Norman Borlaug Centre for Plant Science Research, Institute of Genetic Engineering, De Montfort University, 2232 Kostinbrod-2, Bulgaria (author for correspondence; fax;(2) Institute of Genetic Engineering, 2232 Kostinbrod-2, Bulgaria;(3) Institute of Phytopathology and Plant Protection, University of Hannover, Germany;(4) Institute of Applied Botany, Department of Phytopathology, University of Hamburg, Marseilerstrasse 7, 20355 Hamburg, Germany;(5) Tobacco Breeding Station, Gotze Delchev, Bulgaria |
| Abstract: | We examined the resistance phenotype of a large number of transgenic tobacco plants originating from 12 commercial (Nicotiana tabacum) cultivars expressing the sense form of the nucleoprotein (N) gene of L3, a Bulgarian isolate of tomato spotted wilt virus (TSWV). The analysis revealed that transgenic plants are completely protected against the homologous L3 isolate of TSWV irrespective of whether or not they contain detectable levels of translational product. The effectiveness of protection against the virus was investigated upon mechanical inoculation under greenhouse conditions and in field trials. Non-segregating resistant lines were selected and the inheritance of the resistance to TSWV was analysed in successive generations (R3–R6). Extensive tests under controlled conditions and two-year field trials proved that the resistance to TSWV is stable in different environments and is a stably inherited trait. |
| Keywords: | transgenic tobacco nucleoprotein gene-mediated resistance TSWV field trials |
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