Structural studies of O-glycosidic oligosaccharide units of dog erythrocyte glycophorin |
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Authors: | Teiko Yamashita Jun-ichiro Murayama Hideo Utsumi Akira Hamada |
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Institution: | School of Pharmaceutical Sciences, Showa University, Hatanodai, Shinagawa-ku, Tokyo 142 Japan |
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Abstract: | Dog glycophorin, the major sialoglycoprotein of dog erythrocyte membranes, contains either N-acetyl- or N-glycolylneuraminic acid, depending upon the strain of dog. Glycolipids also contained the same sialic acid as those found in glycophorin when both materials are prepared from erythrocyte membranes of individual dogs. The O-glycosidic oligosaccharides were released from glycophorin, prepared from individual dogs, by alkaline borohydride treatment, and purified by gel filtration and ion-exchange chromatography. The structures of the reduced oligosaccharides were determined by methylation analysis and gas-liquid chromatography-mass spectrometry. The O-glycosidic oligoscharides identified were one tetrasaccharide - Neu5Ac(2→3)Gal)1→3)Neu5Ac(2→6)GalNAcol - two trisaccharides - Neu5Ac(2→3)Gal1→3)GaINAcol and Gal(1→3)Neu5Ac(2→6)]GalNAcol. |
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Keywords: | Glycophorin Carbohydrate structure (Dog erythrocyte) |
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