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The effect of detergents on immunoprecipitability of lysosomal sphingomyelinase |
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| Authors: | Marianne Driessen Gabriele Weitz Elisabeth M. Brouwer-Kelder Wilma E. Donker-Koopman Johan Bastiaannet Konrad Sandhoff John A. Barranger Joseph M. Tager AndréW. Schram |
| Affiliation: | 1. Laboratory of Biochemistry, University of Amsterdam, P.O. Box 20151, 1000 HD Amsterdam, The Netherlands;2. Institute of Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagkstrasse 1, D-5300 Bonn, F.R.G.;3. Developmental and Metabolic Neurology Branch, National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, MD 20205, U.S.A. |
| Abstract: | Antibodies raised against the soluble form of acid sphingomyelinase from human urine and placenta are able to precipitate about 70% of the sphingomyelinase activity present in preparations of urinary sphingomyelinase. In contrast, no precipitation of sphingomyelinase activity occurs in detergent-containing preparations from placenta or splenic membranes. The formation of immune complexes between the antibodies and urinary sphingomyelinase is inhibited if detergents are added. With the non-ionic detergent Triton X-100 significant inhibition occurs only above the critical micellar concentration of the detergent. With the anionic detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulphonate (Chaps) substantial inhibition is already observed below the critical micellar concentration of the detergent. |
| Keywords: | Detergent effect Immunoprecipitation Sphingomyelinase (Lysosome) Chaps 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulphonate HNP Mops 4-morpholino-propanesulphonic acid |
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