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蛭弧菌J26发酵生产维生素C前体产物的鉴定
引用本文:马成新,焦鹏. 蛭弧菌J26发酵生产维生素C前体产物的鉴定[J]. 微生物学杂志, 1998, 18(3): 27-29
作者姓名:马成新  焦鹏
作者单位:中国科学院沈阳应用生态研究所!110015
摘    要:采用有机溶剂沉淀方法去除J26发酵液中杂蛋白,通过离子交换、活性炭吸附去除发酵液中杂质,最后蒸发浓缩获得无色透明结晶。通过对所获晶体的红外光谱分析、熔点测定、纸层析鉴定,证明所获晶体确为维生素C前体2-酮基-L-古龙酸(2-KGA)。

关 键 词:蛭弧菌  2-酮基-L-古龙酸  维生素C  鉴定

IDENTIFICATION OF VITAMIN C PRECURSOR PRODUCT FERMENTED BY BDELLOVIBRIO J26
Ma Chengxin et al.. IDENTIFICATION OF VITAMIN C PRECURSOR PRODUCT FERMENTED BY BDELLOVIBRIO J26[J]. Journal of Microbiology, 1998, 18(3): 27-29
Authors:Ma Chengxin et al.
Abstract:After removing the impurity protein in Bdellovibrio J26 fermentation broth by ethanol precipitation, treating with 732 ion exchanging resin, and adsorption with active carbon, a colorless transparent crystal was obtained by evaporation. Analysis through infrared spectrum, melting point determination, and paper chromatography, the crystal was identified as 2 - keto - gluconic acid.
Keywords:Bdellovibrio  2 - keto - gluconic acid  vitamin C  identification
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