Differential regulation of protooncogene c-myc expression in rat ventral prostate after castration |
| |
Authors: | Lee J H Sul C K Kim Y K Hwang B D Lim K |
| |
Affiliation: | Department of Biochemistry, College of Medicine, Chungnam National University, Daejeon, Korea. |
| |
Abstract: | To evaluate the possibility that the protooncogene c-myc plays a role in ventral prostate, the effects of castration have been investigated at a beginning of a period by Northern blot hybridization and the levels of c-myc mRNA were also compared with mRNA of androgen-regulated genes, C1 and TRPM-2. Levels of c-myc mRNA in ventral prostate increased with maximal stimulation reached at 6 hours (early induction) and 48 hours (late induction) after castration, respectively. The level of C1 mRNA did not change and TRPM-2 was not detected at early induction of c-myc mRNA after castration. The level of early induction of c-myc mRNA after castration was increased in ventral prostate treated with cycloheximide, but it was almost reduced by actinomycin-D pretreatment. Administration of androgen at the time of castration prevented early induction of c-myc mRNA. These results suggest that protooncogene c-myc is differentially regulated in ventral prostate after castration. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|