两株炭疽芽胞杆菌MLST 新序列型(ST)

【目的】2株炭疽芽胞杆菌(Bacillus anthracis)17003-14和17003-32的多位点序列分型(Multilocussequence typing,MLST)研究。【方法】选取B.anthracis基因组7个常见管家基因位点glpF、gmk、ilvD、pta、pur、pycA和tpi进行PCR扩增、测序,与MLST数据库中的等位基因序列进行比对,确定菌株的序列型(sequence type,ST)。【结果】B.anthracis 17003-14和17003-32的等位基因编号分别为113、31、1、43、1、53、7和113、31、1、43、1、53、37,比对结果显示这2株细菌的等位基因编号组合未见报道。【结论】17003-14和17003-32为新ST菌株,已被MLST数据库确认,注册号(pubMLST id)分别为id-1053和id-1054。

Two new sequence type isolates of Bacillus anthracis by multilocus sequence typing

[Objective]To define the sequence type(ST) isolates of Bacillus anthracis by multilocus sequence typing(MLST).[Methods] Fragments of seven housekeeping genes(glpF,gmk,ilvD,pta,pur,pycA,and tpi) were amplified by PCR using the standard primers as described on the website for MLST of Bacillus and the sequences were compared with existing allele sequences on the MLST website.[Results] Two novel allele combinations of the seven loci were found in two isolates 17003-14 and 17003-32.[Conclusion] Two novel ST isolates of B.anthracis were identified by this study and confirmed by the MLST website,and the pubMLST ids were id-1053 and id-1054.