植物乳杆菌LpT1和LpT2大鼠体内降胆固醇特性

【目的】探讨植物乳杆菌LpT1和LpT2大鼠体内降胆固醇特性。【方法】将高脂血症的大鼠随机分成4组,分别进行灌胃。A、B、C和D组分别灌胃菌株LpT1、菌株LpT2、洛伐他汀和蒸馏水。灌胃28d后,断尾采血,分离血清,分别测定总胆固醇、总甘油三酯、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇的含量并进行肝脏组织切片的制作与电镜观察。【结果】饲喂高脂饲料7d后,成功构建出高脂血症大鼠模型。植物乳杆菌菌株LpT1和阳性对照洛伐他汀降胆固醇效果极其显著(p<0.01),菌株LpT2次之(p<0.05),而阴性对照水几乎无降胆固醇效果。从电镜扫描结果看,植物乳杆菌LpT1和LpT2在大鼠肠道中定植后,能很好的调节肝脏代谢脂类物质朝着正常化趋势发展。【结论】研究结果为进一步明确植物乳杆菌体内降胆固醇机制奠定了良好基础。

Cholesterol-degrading in mice by Lactobacillus plantarum LpT1 and LpT2 in vivo

Objective]To explore the cholesterol-degrading in mice by Lactobacillus plantarum LpT1 and LpT2 in vivo.Methods] The hypercholesterolemia mice were randomly classified into 4 groups: A,B,C and D and fed with strain LpT1,LpT2,lovastatin and distilled water,respectively,and then TC,TG,HDL-C,LDL-C and AI were determined.The tissue slice of liver was made and observed by transmission electron microscope.Results] The hypercholesterolemia mice model was successfully constructed after feeding the hypercholesterol forage for 7 days.Lactobacillus plantarum LpT1 and the positive control lovastatin could significantly degrade the total cholesterol content(p<0.01) after 28 days,higher than Lactobacillus plantarum LpT2(p<0.05).However,the negative control water could not degrade it.The transmission electron microscope result showed that the strains could effectively regulate the lipid metabolism to develop towards the regular trend after they were absorbed into the intestines.Conclusion] The results further laid foundation for studying the cholesterol-degrading mechanism by Lactobacillus plantarum in vivo.