|
|||||
|
|
| 金针菇固体发酵菌丝体次级代谢产物分离鉴定 | |
| 引用本文： | 王雅琪,杨晓丽,宝丽,高昊,姚新生,刘宏伟.金针菇固体发酵菌丝体次级代谢产物分离鉴定[J].菌物学报,2012,31(1):127-132. |
| 作者姓名： | 王雅琪 杨晓丽 宝丽 高昊 姚新生 刘宏伟 |
| 作者单位： | 1. 暨南大学药学院 广东广州510632;中国科学院微生物研究所真菌学国家重点实验室 北京1001 01 2. 中国科学院微生物研究所真菌学国家重点实验室 北京100101 3. 暨南大学药学院 广东广州510632 4. 暨南大学药学院 广东广州510632;暨南大学中药药效物质基础及创新药物研究广东省重点实验室 广东广州510632 |
| 基金项目： | 国家自然科学基金（No. 31000036）；国家重点基础研究发展计划项目（No. 2009CB522300）；中国科学院院方向性项目（No. KSCX2-YW-G-074-04） |
| 摘 要： | 采用硅胶柱色谱、ODS柱色谱、Sephadex LH-20柱色谱、HPLC等分离方法对金针菇大米发酵乙酸乙酯提取物进行分离，根据理化性质和波谱数据鉴定化合物结构：鉴定了4个倍半萜，包括1个新的桉叶烷型倍半萜，3个侧柏烷型倍半萜，分别是flamvelutpenol A(1)，aquaticol(2)，enokipodin C(3)，limacellone(4)。并通过与Rh2(OCOCF3)4络合的方法确定了新化合物flamvelutpenol A(1)和limacellone(4)的绝对构型。其中化合物3具有较好的抗菌活性，对耐甲氧西林金黄色葡萄球菌和枯草芽孢杆菌的MIC值分别为12.5mg/L，25mg/L。且化合物1－4均是首次从该种真菌中分离得到。 |
| 关 键 词： | 食用菌 金针菇 倍半萜 结构鉴定 抗菌活性 |
Isolation and identification of secondary metabolites from the solid culture of Flammulina velutipes |
|
| Authors: | WANG Ya-Qi YANG Xiao-Li BAO Li GAO Hao YAO Xin-Sheng and LIU Hong-Wei |
| Institution: | Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou, Guangdong 510632, China State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou, Guangdong 510632, China;Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou, Guangdong 510632, China Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Jinan University;State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
| Abstract: | The ethyl acetate extracts of the solid culture of Flammulina velutipes was subjected to silica gel,ODS,sephadex LH-20 column chromatography and HPLC prepration.Four sesquiterpenoids have been isolated from the mycelial culture of this mushroom.One novel eudesmane-type sesquiterpenoid together with three known cuparene-type sesquiterpenoids were obtained,including flamvelutpenol A (1),aquaticol (2),enokipodin C (3),and limacellone (4).The absolute configuration of 1 and 4 were determined by forming complex with Rh 2 (OCOCF 3) 4 ] and then applying the bulkiness rule.Compounds 3 showed strong antibacterial activity against methicillin-resistant Staphylococcus aureus and Bacillus subtilis with MIC value 12.5mg/L and 25mg/L.Compounds 1-4 were isolated from the fungus for the first time. |
| Keywords: | edible mushroom Flammulina velutipes sesquiterpenoids structural elucidation antimicrobial activity |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《菌物学报》浏览原始摘要信息 | |
| 点击此处可从《菌物学报》下载免费的PDF全文 | |