| 黑曲霉木聚糖酶的底物特异性和低聚木糖生产 |
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| 引用本文： | 吴克,蔡敬民,张洁,刘斌,居萌,王涛,潘仁瑞. 黑曲霉木聚糖酶的底物特异性和低聚木糖生产[J]. 工业微生物, 2000, 30(1): 18-20 |
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| 作者姓名： | 吴克 蔡敬民 张洁 刘斌 居萌 王涛 潘仁瑞 |
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| 作者单位： | 合肥联合大学,生物工程教研室,合肥,230022 |
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| 基金项目： | 安徽省科技厅自然科学基金，安徽省合肥市科技局资助项目，98241515，，， |
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| 摘 要： | 对黑曲霉（Ａｓｐｅｒｇｉｌｌｕｓ ｎｉｇｅｒ）木聚糖酶进行了纯化研究，结果表明，经Ｓｅｐｈａｄｅｘ Ｇ－１００和ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－５０分离后，获得三个组分，称为Ｘ１、Ｘ２、Ｘ３。它们经ＰＡＧＥ电泳分析均为单一组分。对Ｘ１、Ｘ２、Ｘ３的相关性质，特别是底物特异性也作了研究，纯酶Ｘ３组分或部分纯化的酶可用于生产低聚木糖，产品得率为１０％。
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| 关 键 词： | 黑曲霉 木聚糖酶 低聚木糖 底物特异性 |
Substrate specificities of xylanases from Aspergillus niger and its relationship of xylooligosaccharide production |
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| WU Ke,CAI Jing-min,ZHANG Jie,LIU Bin,JU Meng,WANG Tao,PAN Ren-rui. Substrate specificities of xylanases from Aspergillus niger and its relationship of xylooligosaccharide production[J]. Industrial Microbiology, 2000, 30(1): 18-20 |
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| Authors: | WU Ke CAI Jing-min ZHANG Jie LIU Bin JU Meng WANG Tao PAN Ren-rui |
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| Abstract: | Xylanases purification from Aspergillus niger were studied.Three kinds of xylanases,X1,X2 and X3,were obtained by means of isolation from Sephadex G?100 and DEAE?Sephadex A?50.The pure xylanases showed homogeneous with PAGE.The relative properties of xylanases were studied,especially their substrate specificities.Hydrolysis of corncob hemicellulose with xylanase X3 of partially purified enzyme could be used for xylooligosaccharide production.The recovery was 10%. |
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| Keywords: | Aspergillus niger Xylanase xylooligosaccharide |
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