Expression of recombinant protoporphyrinogen oxidase influences growth and morphological characteristics in transgenic rice |
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| Authors: | Sunyo Jung Jung Sung Chung Sang-Uk Chon Yong In Kuk Hee Jae Lee Ja Ock Guh Kyoungwhan Back |
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| Institution: | (1) Department of Biotechnology, Biotechnology Research Institute, Korea;(2) Institute of Agricultural Science and Technology, Chonnam National University, Gwangju, 500-757, Korea;(3) Biotechnology Industrialization Center, Dongshin University, Naju, 520-714, Korea;(4) School of Plant Science, Seoul National University, Seoul, 151-742, Korea |
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| Abstract: | Transgenic rice plants expressing a Bacillus subtilis protoporphyrinogen oxidase (Protox), the last shared enzyme of the porphyrin pathway, in the cytoplasm (C89) or the plastids
(P72) were compared with wild-type rice plants in their growth characteristics. Production of tiller buds 18 d after seeding
was more profuse in transgenic plants than in wild-type plants, especially in plastid-targeted plants. Transgenic plants had
12–27% increase in tiller number and 17–33% increase in above-ground biomass compared with wild-type plants 4 and 8 weeks
after transplanting of 2-week-old rice seedlings, demonstrating that tiller production and above-ground biomass correlate
with each other. Cytoplasm-expressed and plastid-targeted transgenic plants also had a distinct phenotypic characteristic
of narrower and more horizontal leaves than wild-type plants. Phenotypic and anatomical characteristics of the transgenic
plants were clearly different from wild-type plants, indicating that regulation of porphyrin biosynthesis by expression of
B. subtilis Protox in rice influences morphological characteristics of plant growth as well as biomass. |
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| Keywords: | Bacillus subtilis Growth Porphyrin Protoporphyrinogen oxidase Rice Tiller |
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