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| 聚天冬氨酸-甲硝唑纳米前药制备、表征及抗虫活性的初步研究 | |
| 引用本文： | 戚晓红,陈汐敏,冯振卿,管晓虹,吴 军,陈 强,阚延静,童 华.聚天冬氨酸-甲硝唑纳米前药制备、表征及抗虫活性的初步研究[J].生物化学与生物物理进展,2009,36(8):1056-1063. |
| 作者姓名： | 戚晓红 陈汐敏 冯振卿 管晓虹 吴 军 陈 强 阚延静 童 华 |
| 作者单位： | 1. 南京医科大学卫生部抗体技术重点实验室,南京,210029;南京医科大学病理与病理生理学系,南京,210029 2. 南京医科大学卫生部抗体技术重点实验室,南京,210029 3. 南京大学表面和界面化学工程技术研究中心,南京,210093 4. 南京市妇幼保健院,南京,210004 |
| 基金项目： | 南京市卫生局科技发展专项基金资助 (D:ZKX 0103) |
| 摘 要： | 实验以聚天冬氨酸(PASP)为载体，以甲硝唑为模型药物，用较温和简便的方法制备了新型聚天冬氨酸-甲硝唑(PASP-MTI)纳米前药．采用红外光谱及核磁氢谱分析、透射电镜观察、激光粒度、透析和紫外分光光度测定、MTT比色、荧光显微镜和流式细胞术等方法，观察到甲硝唑以酯键方式键合于聚天冬氨酸高分子链上．PASP-MTI纳米前药呈球型，平均粒径404.8 nm，载药量30%，体外释药明显延缓；MTT结果显示，PASP-MTI显著提高甲硝唑对滴虫的抑杀作用，经纳米甲硝唑作用后，部分滴虫胞核出现染色质浓集、核固缩、核碎裂等一系列凋亡改变．流式细胞术检测可见凋亡峰，凋亡率由对照组的11.5%上升到纳米前药组的35.69%．上述实验结果表明：PASP是一种非常有潜力的前药载体，制得的新型PASP-MTI纳米前药具有载药量高、缓释和杀虫作用强等特点，可能的杀虫机制除与高聚物纳米前药促吞噬作用有关外，还与诱导滴虫凋亡有关． |
| 关 键 词： | 聚天冬氨酸，甲硝唑，纳米粒，前药，缓释，阴道毛滴虫，凋亡 |
| 收稿时间： | 2008/12/18 0:00:00 |
| 修稿时间： | 2009/3/15 0:00:00 |
Preparation, characterization and preliminary trichomonacidal effect of poly aspartic acid-metronidazole nanoprodrug |
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| QI Xiao-Hong,CHEN Xi-Min,FENG Zhen-Qing,GUAN Xiao-Hong,WU Jun,CHEN Qiang,KAN Yan-Jing and TONG Hua.Preparation, characterization and preliminary trichomonacidal effect of poly aspartic acid-metronidazole nanoprodrug[J].Progress In Biochemistry and Biophysics,2009,36(8):1056-1063. | |
| Authors: | QI Xiao-Hong CHEN Xi-Min FENG Zhen-Qing GUAN Xiao-Hong WU Jun CHEN Qiang KAN Yan-Jing and TONG Hua |
| Institution: | The Key Laboratory Antibody Technology of State Ministry of Health, Nanjing Medical University, Nanjing 210029, China; Department of Pathology and Pathophysiology, Nanjing Medical University, Nanjing 210029, China;The Key Laboratory Antibody Technology of State Ministry of Health, Nanjing Medical University, Nanjing 210029, China;The Key Laboratory Antibody Technology of State Ministry of Health, Nanjing Medical University, Nanjing 210029, China; Department of Pathology and Pathophysiology, Nanjing Medical University, Nanjing 210029, China;The Key Laboratory Antibody Technology of State Ministry of Health, Nanjing Medical University, Nanjing 210029, China;Center of Research on Surface and Interface Chemical Engineering and Technology, Nanjing University, Nanjing 210093, China;Center of Research on Surface and Interface Chemical Engineering and Technology, Nanjing University, Nanjing 210093, China;Nanjing Maternal and Child Health Hospital, Nanjing 210004, China;Nanjing Maternal and Child Health Hospital, Nanjing 210004, China |
| Abstract: | |
| Keywords: | poly aspartic acid metronidazole nanoparticle prodrug prolonged release T vaginalis apoptosis |
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