Effect of temperature on plasma membrane and tonoplast ion channels in Arabidopsis thaliana |
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Authors: | Roberta Colombo Raffaella Cerana |
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Affiliation: | Dipto di Biologia, Univ. di Milano, Via Celoria 26, 1–20133 Milano, Italy. |
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Abstract: | The temperature dependence of the activity of ion channels was investigated, by means of the patch-clamp technique in the 'whole-cell' configuration, using protoplasts and vacuoles isolated form Arabidopsis thaliana L. cultured cells. The effect of temperature changes in the range 11–22°C was tested on the hyperpolarization and depolarization-activated K+ currents in the plasma membrane and on the hyperpolarization-activated K− currents in the tonoplast (vacuolar membrane). All 3 kinds of currents were unaffected by increasing temperature up to 15°C and were activated between 15 and 20°C. |
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Keywords: | Arabidopsis thaliana ion channels patch-clamp plasma membrane tonoplast vacuolar membrane |
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