The effect of pleiotrophin signaling on adipogenesis |
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Authors: | Gu Dayong Yu Bing Zhao Chen Ye Wenbin Lv Qing Hua Zhong Ma Jiangan Zhang Yaou |
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Affiliation: | Life Science Division, Graduate School at Shenzhen, Tsinghua University, Room 407, Building L, Tsinghua Campus, University Town, Shenzhen, Guangdong 518055, PR China. |
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Abstract: | Pleiotrophin (PTN) plays diverse roles in cell growth and differentiation. In this investigation, we demonstrate that PTN plays a negative role in adipogensis and that glycogen synthase kinase 3beta (GSK-3beta) and beta-catenin are involved in the regulation of PTN-mediated preadipocyte differentiation. Knocking down the expression of PTN using siRNA resulted in an increase in phospho-GSK-3beta expression, and the accumulation of nuclear beta-catenin, which are critical downstream signaling proteins for both the PTN and Wnt signaling pathways. Our investigation suggests that there is a PTN/PI3K/AKT/GSK-3beta/beta-catenin signaling pathway, which cross-talks with the Wnt/Fz/GSK-3beta/beta-catenin pathway and negatively regulates adipogenesis. |
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Keywords: | PTN, pleiotrophin GSK-3β, glycogen synthase kinase 3β PPARγ, peroxisome proliferator-activated receptor γ M, methylisobutylxanthine D, dexamethasone I, insulin RTPT, receptor protein tyrosine phosphatase Fz, frizzled receptor LRP, low density lipoprotein receptor-related protein TCF/LEF, T-cell factor/lymphoid-enhancing factor PI3K, phosphoinositide 3-kinase GRE, glucocorticoid response element |
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