Supercoil-induced Z-DNA formation within 5'-end of chicken myb proto-oncogene |
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| Authors: | J Vlach M Dvorak P Bartunek V Pecenka M Travnicek J Sponar |
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| Affiliation: | Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague. |
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| Abstract: | We have analyzed the recently sequenced and characterized 2.9 kb fragment derived from the 5'-end of chicken myb proto-oncogene with respect to structural perturbations induced by DNA supercoiling. Within the first intron a 50 bp sequence stretch was localized, starting approximately 450 nucleotides downstream from putative ATG initiation codon, which forms a non-B-DNA structure. Fine mapping with structural probes revealed the three adjacent regions with imperfect purine-pyrimidine alternation creating together relatively long Z-forming tract, parts of which may undergo a B-Z DNA transition at different superhelical densities. |
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