Biochemical characterization of H9/25, an allospecificity encoded by the Ly-6 region |
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Authors: | Dr Fumio Takei |
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Institution: | (1) Department of Pathology, University of British Columbia and the Terry Fox Laboratory, British Columbia Cancer Research Centre, Vancouver, B. C., Canada;(2) Present address: Terry Fox Laboratory, British Columbia Cancer Research Centre, 601 West 10th Avenue, V5Z 1L3 Vancouver, B. C., Canada |
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Abstract: | H9/25, an allospecificity encoded by the Ly-6 region, was biochemically characterized. It was sensitive to pepsin and heat treatment, but was resistant to periodate oxidation. Its apparent molecular weight was approximately 12 000 daltons by gel filtration. The antigenic molecule was partially purified by gel filtration and antibody affinity chromatography. The partially purified antigen molecule was radioiodinated, immunoprecipitated with monoclonal antibody H9/25, and analyzed by SDS-polyacrylamide gel electrophoresis. The autoradiograph showed the molecular weight of H9/25 to be approximately 15000 daltons under reducing conditions. These results indicate that H9/25 is a protein with a single polypeptide chain of 12000–15000 daltons molecular weight, and the antigenic specificity is carried by a peptide but not a carbohydrate moiety. |
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