Viability of glycerol-preserved and cryopreserved anuran skin |
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Authors: | Scott Willens Michael K Stoskopf Linda D Martin Gregory A Lewbart |
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Institution: | (1) Environmental Medicine Consortium College of Veterinary Medicine, North Carolina State University, 27606 Raleigh, North Carolina;(2) Department of Clinical Sciences College of Veterinary Medicine, North Carolina State University, 27606 Raleigh, North Carolina;(3) Department of Molecular Biomedical Sciences College of Veterinary Medicine, North Carolina State University, 27606 Raleigh, North Carolina;(4) USAMRICD, 3100 Ricketts Point Road, APG-EA, 21010-5400 Maryland |
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Abstract: | Summary Anurans are important animal models for studying the effects of anthropogenic chemical contamination of the environment. Two-compartment
Teflon flow-through diffusion cells can be used to study percutaneus absorption of xenobiotics across harvested skin. However,
such an approach currently necessitates that skin be harvested just before experimentation, a requirement that calls for the
continuous growth and housing of living animals. The ability to preserve and store skin would allow more efficient use of
animals and more flexibility in experimental design. To this end, we examined the viability of harvested anuran skin stored
under various protocols consistent with current practices of mammalian skin preservation. Skin from the American bullfrog
maintained 80–85% viability after 28 d, whereas viability of skin from the marine toad was only maintained for 7–10 d. |
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Keywords: | Bufo marinus Rana catesbeiana trypan |
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