Effects of thidiazuron and N6-benzylaminopurine on shoot regeneration of Phalaenopsis |
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| Authors: | Yongqin Chen and Chitrapan Piluek |
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| Affiliation: | (1) Department of Horticulture, Kasetsart University, 10900 Bangkok, Thailand;(2) Present address: Plant Physiology Laboratory, Guizhou Agricultural College, 550025 Guiyang, Guizhou Province, The Peoples' Republic of China |
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| Abstract: | Adventitious bud formation from the vegetative buds of the flower stalks of Phalaenopsis occurred on Vacin and Went medium with 15% coconut water and 5 to 40  M thidiazuron (TDZ) or 40 M N6-benzylaminopurine. The highest efficiency of induction was achieved with 5 or 10 M TDZ. Adventitious buds developed into shoots on VWC medium. TDZ was more effective than BAP in stimulating the axillary buds of intact shoots to develop. Regenerated shoots rooted after about two months of culture on VWC medium with 1% sucrose. Shoot tips excised from the regenerated shoots initiated protocorm-like bodies after two months of culture on VWC medium.Abbreviations VWC medium Vacin and Went medium with 15% (by volume) coconut water - TDZ thidiazuron - BAP N6-benzylaminopurine - Plbs protocorm-like bodies |
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| Keywords: | adventitious buds aseptic culture benzylaminopurine flower stalks Phalaenopsis protocorm-like bodies thidiazuron |
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