Late replication patterns in adult and embryonic mice carrying Searle's X-autosome translocation |
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Authors: | Christine M. Disteche Eva M. Eicher Samuel A. Latt |
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Affiliation: | 1. Genetics Division and Mental Retardation Center, Children''s Hospital Medical Center, and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA;2. Jackson Laboratory, Bar Harbor, ME 04609, USA |
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Abstract: | Bromodeoxyuridine-dye technique analysis of X chromosome DNA synthesis in female adult and fetal mice carrying the balanced form of the T(X; 16) 16H translocation demonstrated that the structurally normal X chromosome was late replicating (and hence presumably inactive) in 93% of the adult cells and 99% of the 9-day embryo cells, with the X16 chromosome late replicating in the remaining cells. We conclude from these results that in T16H/+ females either there is preferential inactivation of the normal X chromosome or that, if inactivation is random, cell selection takes place before 9 days of development. Two 9-day female embryos with an unbalanced karyotype were also studied; both had two late-replicating chromosomes in most of their cells, one being the chromosome 16X, the other a normal X chromosome. These results, together with the presence of a late-replicating X16 chromosome in T16H/+ adult and fetal mice, support the concept that more than one inactivation center is present on the X chromosome of the mouse because the X16 and the 16x chromosomes can be late replicating. |
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