Molecular cloning and expression in Escherichia coli K-12 of chromosomal genes determining the O antigen of an E. coli O2: K1 strain |
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| Authors: | Brian L Neal George C Tsiolis Michael W Heuzenroeder Paul A Manning Peter R Reeves |
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| Institution: | Department of Microbiology, University of Sydney, Australia;Department of Microbiology and Immunology, University of Adelaide, Australia |
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| Abstract: | Abstract: The rfb gene cluster which determines the biosynthesis of the O2 O antigen has been cloned from an Escherichia coli O2: K1 strain isolated from a case of septicaemia in chickens. The region required for expression of the O antigen in E. coli K-12 was localised to a 10.7 to 14.15-kb segment which was shown to be chromosomal in origin with a close linkage to the gnd and his genetic loci. |
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| Keywords: | Escherichia coli rfb O2 Antigen O Lipopolysaccharide Serotype |
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