Effect of fractions of horse and fetal bovine serum on neoplastic conversion of C3H mouse cells in tissue culture |
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Authors: | Floyd M Price R Raymond Gantt Virginia J Evans |
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Institution: | (1) Tissue Culture Section, Laboratory of Biology, National Cancer Institute, National Institutes of Health, 20014 Bethesda, Maryland |
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Abstract: | Summary Cultures derived from C3H/He mouse embryos were grown in medium NCTC 135 supplemented with horse serum, fetal bovine serum,
or various combinations of large and small molecule fractions of horse and fetal bovine serum. Cultures in medium NCTC 135
alone or in medium 135 supplemented with the small molecule fraction of either horse or fetal bovine serum did not grow as
continuous long term lines. The best growth was obtained when the cultures were in medium containing the large molecule fraction
of fetal bovine serum either alone or in combination with a small molecule fraction. Cells grown in the presence of the low
molecular weight fraction of horse serum invariably produced tumors on injection into syngeneic animals. Cells in the small
molecular weight fraction of fetal bovine serum combined with the large molecular weight fraction of horse serum produced
tumors after a prolonged period in vitro. *** DIRECT SUPPORT *** A00S8010 00003 |
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