肝癌细胞与内皮细胞的粘附力学特性研究

采用细胞同步技术和微管吸吮技术,从细胞周期的角度研究不同细胞周期肝癌细胞(hepatocellularcarcinoma cells,HCC)与脐静脉内皮细胞(HUVEC)的粘附力学特性。结果表明,未同步化肝癌细胞各周期时相的细胞百分比为:G_0/G_1期,53.51％;G_2/M期,11.01％;S期,35.48％。采用胸腺嘧啶脱氧核苷、秋水仙碱顺序阻断和胸腺嘧啶脱氧核苷双阻断后释放培养的方法可分别获得G_1期和S期的肝癌细胞,其平均同步率分别为69.02％和96.50％。G_1期肝癌细胞与人脐静脉内皮细胞的粘附力比S期相应值明显降低(P<0.01),与未同步化肝癌细胞组比较也得到同样结果,而S期与未同步化肝癌细胞组的粘附力值无明显差别。肝癌细胞与脐静脉内皮细胞的粘附力随着粘附时间的变化而变化,在30～60min内迅速增长,60min之后维持在较稳定的水平,即300×10~(10)N左右。提示:在肝癌细胞与内皮细胞的粘附过程中,S期细胞可能起的作用更大;肝癌细胞和内皮细胞上粘附分子表达呈现时间效应,从而体现出粘附和去粘附的行为特征。

STUDIES ON THE ADHESIVE MECHANICAL PROPERTIES BETWEEN HEPATOCELLULAR CARCINOMA CELLS AND ENDOTHELIAL CELLS

The purpose of the study is to investigate the adhesive mechanical properties between hepatocellular carcinoma cells (HCC) in different cell cycle phase and human umbilical vein endothelial cells (HUVECs) by cellular biology and cellular mechanics, using micropipette aspiration and cell synchronization technique. It showed that the percentage of different phases of general HCC cells cycle are: G0/G1 phase 53.51%, G2/M phase 11.01%, S phase 35.48%; the synchronous G, and S phase HCC cells were achieved through thymine-2-desoxyriboside, colchicines sequential blockage method and double thymine-2-desoxyriboside blockage method, the synchronous rates of G1 and S phase amount to 69.02% and 96.50%, respectively. The adhesive forces changed with the variations of adhesive time and presented behavior characteristics of adhesion and de-adhesion; S phase SMMC-7721 cells have higher adhesion forces to HUVECs than G1 phase cells(P<0.01), so it can be suggested S phase cells may play main roles in its adhesion to endothelial cells.