Construction of SSR-based chromosome map in bunching onion (Allium fistulosum) |
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Authors: | Hikaru Tsukazaki Ken-ichiro Yamashita Shigenori Yaguchi Shinichi Masuzaki Hiroyuki Fukuoka Junichi Yonemaru Hiroyuki Kanamori Izumi Kono Tran Thi Minh Hang Masayoshi Shigyo Akio Kojima Tadayuki Wako |
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Institution: | National Institute of Vegetable and Tea Science (NIVTS), NARO, 360 Ano-Kusawa, Tsu, Mie, 514-2392, Japan. |
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Abstract: | We have constructed a linkage map of bunching onion (Allium fistulosum L., 2n = 16) using an F(2) population of 225 plants. The map consists of 17 linkage groups with 212 bunching onion SSR markers and 42 bulb onion (A. cepa L.) SSR, InDel, CAPS or dCAPS markers, covering 2,069 cM. This is the first report of a linkage map mainly based on SSR markers in the genus Allium. With the 103 anchor markers 81 bunching onion SSRs, 11 bulb onion SSRs and 11 bulb onion non-SSRs (1 InDel, 9 CAPSs and 1 dCAPS)] whose chromosome assignments were identified in A. cepa and/or A. fistulosum, via the use of several kinds of Allium alien addition lines, 16 of the 17 linkage groups were connected to the 8 basic chromosomes of A. cepa. |
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