应用PCR-DGGE法评价石斛多糖对肠道微生态失调的调节作用

目的 从微生态学角度研究石斛多糖治疗肠道微生态失调小鼠的作用及初步机制.方法 盐酸林可霉素灌胃制备肠道菌群失调小鼠模型,应用石斛多糖进行治疗,同时设正常对照组、丽珠肠乐组、阴性对照组,给药7d后处死小鼠,应用PCR-DGGE法检测肠道菌群丰富度、血清IL-2.结果 应用盐酸林可霉素灌胃3d后,小鼠肠道菌群丰富度、血清IL-2降低,持续治疗7d后,石斛多糖治疗小鼠肠道菌群丰富度、血清IL-2增高.结论 石斛多糖具有扶植肠道正常菌群生长,调整菌群失调,提高机体免疫力的作用.

Regulatory effects of dendrobium polysaccharides on intestinal dysbiosis: an evaluation by PCR-DGGE

Objective To explore the role and preliminary mechanism of Dendrobium Polysaccharides in treating mice intestinal microflora imbalance from a micro-ecological perspective.Methods Lincomycin hydrochloride was orally administered to induce intestinal microflora imbalance in the mice,after which the mice were treated with Dendrobium Polysaccharides.At the same time,the mice were randomly divided into normal control group,Oral Live Bifodobacterium preparation group and negative control group.After 7 days of treatment with various medicines,the mice were sacrificed and the PCR-DGGE and ELISA were used to detect intestinal flora richness and serum IL-2 respectively.Results After 3 days of oral administration of Lincomycin hydrochloride,there was a decrease in the intestinal flora richness and serum IL-2 of the mice.However,after 7 days of continuous treatment with Dendrobium Polysaccharides,an increase in the intestinal flora richness and serum IL-2 were observed.Conclusion Dendrobium Polysaccharide fosters the growth of normal intestinal flora,has a regulatory effect on intestinal flora,and improves immunity.