荆芥体外抗阴道毛滴虫作用机制的实验研究

目的探讨荆芥体外杀灭阴道毛滴虫的作用机制。方法将浓度为1∶4的荆芥水提液作用于体外培养的阴道毛滴虫,并采用透射电镜观察经药物作用2 h和4 h后阴道毛滴虫的超微结构变化。结果荆芥作用后阴道毛滴虫多聚核糖体解聚,粗面内质网脱颗粒,胞质内可见大量空泡;随药物作用时间延长,核膜不完整,核质变疏松;最终,胞膜破损,内容物外溢,虫体死亡。结论荆芥可破坏阴道毛滴虫的内膜系统,具有较强的抗滴虫作用。

Mechanism of killing action by Herba schizonepetae on Trichomonas vaginalis in vitro

Objective To explore the mechanism of killing action by Herba schizonepetae on Trichomonas vaginalis in vitro.Method 1:4 concentration of Herba schizonepetae water extract(HSWE) was added to the culture media of T.vaginalis in vitro.After treated with HSWE for 2 h and 4 h respectively,the ultrastructural changes of T.vaginalis were observed by transmission electronic microscope.Result After treated with HSWE,T.vaginalis exhibited depolymerization of polyribosomes,degranulation of rough surfaced endoplasmic reticula and increment of vacuoles in cytoplasm.With the prolongation of drug action time,incomplete nuclear membranes and raritas karyoplasm appeared.Eventually nuclear membranes ruptured,their cellular content overflew,and then parasites died.Conclusion Herba schizonepetae can destroy the endomembrane system of Trichomonas and thus exhibits a relatively potent killing effect on T.vaginalis.