Monoclonal Antibodies Specific for Non-Derivatized Gibberellins I. Preparation of Monoclonal Antibodies against GA4 and Their Use in Immunoaffinity Column Chromatography |
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| Authors: | Nakajima Masatoshi; Yamaguchi Isomaro; Nagatani Akira; Kizawa Satoru; Murofushi Noboru; Furuya Masaki; Takahashi Nobutaka |
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| Institution: | 1Department of Agricultural Chemistry, The University of Tokyo Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113 Japan
2Frontier Research Programs, RIKEN Hirosawa 2-1, Wako-shi, Saitama, 350-01 Japan |
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| Abstract: | Cell lines producing monoclonal antibodies specific for non-derivatizedgibberellins (GAs) were prepared from spleen cells of a mouseimmunized with an immunogen that carried 16-substituted GA4as a hapten. The conditions for association and dissociationof antibody-hapten complexes were examined to determine themost effective procedure for immunoaffinity chromatography.The final procedure adopted was proven to be very effectiveby its application to the analysis of GAs in the anthers ofrice and immature seeds of Phaseolus vulgaris
4Present address: Department of Agricultural Chemistry, UtsunomiyaUniversity, Mine-machi 350, Utsunomiya, 321 Japan (Received August 8, 1990; Accepted March 7, 1991) |
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