Construction of a homologous selectable marker gene for Lentinula edodes transformation |
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| Authors: | Irie Toshikazu Sato Toshitsugu Saito Kumiko Honda Yoichi Watanabe Takashi Kuwahara Masaaki Enei Hitoshi |
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| Institution: | Iwate Biotechnology Research Center, Kitakami, Iwate, Japan. |
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| Abstract: | We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it. The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 microg vector DNA). Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold. |
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