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| 小鲵科线粒体16S rRNA基因序列分析及其系统发育 | |
| 引用本文： | 李悦,吴敏,王秀玲. 小鲵科线粒体16S rRNA基因序列分析及其系统发育[J]. 动物学报, 2004, 50(3): 464-469 |
| 作者姓名： | 李悦 吴敏 王秀玲 |
| 作者单位： | 1. 浙江大学生命科学学院,杭州,310029;濒危野生动物保护遗传与繁殖教育部重点实验室,杭州,310029 2. 浙江大学生命科学学院,杭州,310029 3. 新疆师范大学生物系,乌鲁木齐,830054 |
| 摘 要： | To study the phylogeny of Hynobiidae, we amplified DNA fragments of 470 bp 16S ribosomal RNA (16S rRNA) gene on mitochondrial DNA from Ranodon sibiricus and Ranodon tsinpaensis. PCR products were cloned into PMD18 T vector after purification. These sequences were determined and deposited in the GenBank (accession numbers: AY373459 for Ranodon sibiricus, AY372534 for Ranodon tsinpaensis). By comparing the nucleotide differences of 16S ribosomal RNA sequences among Liua shihi, Pseudohynobius flavomaculatus and Batrachuperus genus from GenBank database, we analyzed the divergences and base substitution among these sequences with the MEGA software. The molecular results support that B. tibetanus, B. pinchonii and B. karlschmidti are classified into three valid species. Liua shihi has closer phylogenetic relationships to Ranodon tsinpaensis than to other species. More our results reveal that Pseudohynobius flavomaculatus is not a synonym of Ranodon tsinpaensis. [Acta Zoologica Sinica 50 (3) : 464 - 469,2004]. |
| 关 键 词： | 小鲵科 16S核糖体RNA 分子系统学 |
Phylogenetic relationships of Hynobiidae based on sequences of mitochondrial 16S ribosomal RNA gene |
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| LI Yue ,,WU Min ,WANG Xiu Ling College of Life Science,Zhejiang University,Hangzhou ,China The Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals,Ministry of Education,Hangzhou ,China. Phylogenetic relationships of Hynobiidae based on sequences of mitochondrial 16S ribosomal RNA gene[J]. Acta Zoologica Sinica, 2004, 50(3): 464-469 | |
| Authors: | LI Yue WU Min WANG Xiu Ling College of Life Science Zhejiang University Hangzhou China The Key Laboratory of Conservation Genetics Reproductive Biology for Endangered Wild Animals Ministry of Education Hangzhou China |
| Affiliation: | LI Yue 1,2,WU Min 1**,WANG Xiu Ling 3 1 College of Life Science,Zhejiang University,Hangzhou 310029,China 2 The Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals,Ministry of Education,Hangzhou 310029,China 3 Department of Biology,Xinjiang Normal University,Urumqi 830054,China |
| Abstract: | To study the phylogeny of Hynobiidae, we amplified DNA fragments of 470 bp 16S ribosomal RNA (16S rRNA) gene on mitochondrial DNA from Ranodon sibiricus and Ranodon tsinpaensis. PCR products were cloned into PMD18 T vector after purification. These sequences were determined and deposited in the GenBank (accession numbers: AY373459 for Ranodon sibiricus, AY372534 for Ranodon tsinpaensis). By comparing the nucleotide differences of 16S ribosomal RNA sequences among Liua shihi,Pseudohynobius flavomaculatus and Batrachuperus genus from GenBank database,we analyzed the divergences and base substitution among these sequences with the MEGA software. The molecular results support that B tibetanus, B pinchonii and B karlschmidti are classified into three valid species. Liua shihi has closer phylogenetic relationships to Ranodon tsinpaensis than to other species. More our results reveal that Pseudohynobius flavomaculatus is not a synonym of Ranodon tsinpaensis. . |
| Keywords: | Hynobiidae 16S ribosomal RNA Molecular phylogeny |
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