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Anti-oxidant effect of ascorbic and dehydroascorbic acids in hippocampal slice culture |
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| Authors: | Kim Eun Jin Won Ran Sohn Jin-Hun Chung Myung-Ae Nam Taick Sang Lee Hye-Jung Lee Bae Hwan |
| Affiliation: | a Department of Physiology, Brain Research Institute, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, C.P.O. Box 8044, Seoul 120-752, Republic of Korea b Department of Psychology, Brain Research Institute, Chungnam National University, Daejeon, Republic of Korea c Medical Information Convergence Service Research Team, IT Convergence Services Future Technology Research Group, Electronics and Telecommunications Research Institute, Daejeon, Republic of Korea d Acupuncture and Meridian Science Research Center, Kyung Hee University, Seoul, Republic of Korea |
| Abstract: | Ascorbic acid (AA) and dehydroascorbic acid (DHA) have been shown to have protective effects as anti-oxidants in experimental neurological disorder models such as stroke, ischemia, and epileptic seizures. The present study was conducted to examine the protective effects of AA and DHA on kainic acid (KA) neurotoxicity using organotypic hippocampal slice cultures. After 12 h KA treatment, significant delayed neuronal death was detected in the CA3, but not the CA1, region. Pretreatment with intermediate doses of AA and DHA significantly prevented cell death and inhibited reactive oxygen species (ROS) level, and mitochondrial dysfunction in the CA3 region. In contrast, pretreatment with low or high doses of AA or DHA was not effective. These data suggest that pretreatment with both AA and DHA has dose-dependent neuroprotective effects on KA-induced neuronal injury through inhibiting ROS generation and mitochondrial dysfunction. |
| Keywords: | Anti-oxidants Ascorbic acid Dehydroascorbic acid Kainic acid Reactive oxygen species Organotypic hippocampal slice culture |
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