Long-term dark rearing induces permanent reorganization in retinal circuitry |
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Authors: | Giovannelli Aldo Di Marco Stefano Maccarone Rita Bisti Silvia |
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Institution: | a Dipartimento di Medicina Sperimentale, Università di L’Aquila, via Vetoio, Coppito 2, L’Aquila 67100, Italy b Dipartimento di Scienze e Tecnologie Biomediche, Università di L’Aquila, Coppito 2, L’Aquila 67100, Italy c Istituto Nazionale di Biostrutture e Biosistemi (INBB), Roma 00100, Italy |
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Abstract: | Recent data challenged the assumption that light has little effect on retina development. Here, we report evidence that dark rearing permanently changes the synaptic input to GCs. A reduced spontaneous postsynaptic currents (SPSCs) frequency was found in retinal GCs from rats born and raised in the dark for three months. Glutamate antagonists (CNQX and AP-5) reversibly reduced SPSCs frequency in control and dark-reared (DR) retinae. The GABA antagonist picrotoxin (PTX) reduced SPSCs frequency in control retinas, but increased SPSCs frequency in DR, mainly by presynaptic action on excitatory currents. In DR animals exposed to normal cyclic light for 3 months, SPSCs frequency remained lower then in control rats and increased following PTX, suggesting that long-term dark rearing induces permanent modifications of the retinal circuitry. Our results strongly support the idea that light stimulation plays a role in establishing normal synaptic input to GCs. |
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Keywords: | IPL inner plexiform layer PTX picrotoxin RGCs retinal GCs BCs retinal BCs sEPSCs spontaneous excitatory postsynaptic currents sIPSCs spontaneous inhibitory postsynaptic currents SPSCs spontaneous postsynaptic currents T0 5 decay time |
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