Effects of PAX3-FKHR on malignant phenotypes in alveolar rhabdomyosarcoma |
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Authors: | Kikuchi Ken Tsuchiya Kunihiko Otabe Osamu Gotoh Takahiro Tamura Shinichi Katsumi Yoshiki Yagyu Shigeki Tsubai-Shimizu Satoko Miyachi Mitsuru Iehara Tomoko Hosoi Hajime |
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Institution: | Department of Pediatrics, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramchi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan |
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Abstract: | The malignancy of alveolar rhabdomyosarcoma (ARMS) has been linked to expression of the PAX3-FKHR chimeric gene. To understand the effect of this gene, we used RNAi to knock down its expression (without affecting the expressions of either PAX3 or FKHR) in human ARMS cell lines. Down-regulating PAX3-FKHR caused (a) tumor cells to accumulate in the G1 phase, inhibiting the rate of cellular proliferation, (b) a reduction in the levels of the MET, reducing cell motility stimulated by HGF, and (c) induction of the myogenic differentiation gene, myogenin, and muscle differentiation (morphologic change and the expression of muscle specific proteins, desmin, and myosin heavy chain). These results suggest that PAX3-FKHR in ARMS cells promotes malignant phenotypes such as proliferation, motility, and to suppress differentiation. |
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Keywords: | Rhabdomyosarcoma PAX3-FKHR siRNA MET Myogenin Cell cycle progression Motility Muscle differentiation |
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