Common polymorphisms in CYP1A1, GSTM1, GSTT1, GSTP1 and XPD genes and endogenous DNA damage |
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Authors: | Marta Wlodarczyk Grazyna Nowicka |
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Institution: | (1) Department of Nutrigenomics, National Food and Nutrition Institute, Warsaw, Poland;(2) Department of Pharmacogenomics, Medical University of Warsaw, Warsaw, Poland |
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Abstract: | Endogenous DNA damage levels were analyzed in relation to polymorphisms in genes encoding phase I detoxifying enzyme—CYP1A1,
phase II detoxifying enzymes—GSTM1, GSTT1, GSTP1 and enzyme involved in nucleotide excision repair-XPD. The study group consisted
of 220 healthy non-smoking volunteers; 90 men and 130 woman, 25–60 years old (44 ± 10 years). The level of DNA damage (% DNA
in tail) was evaluated by alkaline comet assay. The genetic variants were determined by restriction fragment length polymorphism
PCR. The highest level of DNA damage (6.7%) was found in carriers of both: AA variant of XPD gene and M1 null variant of GSTM1
gene. The lowest level of DNA breaks (3.7%) was associated with the genotype GSTP1-AA/GSTM1 (+). |
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