Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy |
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Authors: | Yang Lijun Kajiura Hiroyuki Suzuki Kazuya Hirose Sakiko Fujiyama Kazuhito Takaiwa Fumio |
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Affiliation: | a Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan b The International Center for Biotechnology, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan |
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Abstract: | As an alternative approach to conventional allergen-specific immunotherapy, transgenic rice seed expressing a major house dust mite (HDM) allergen, Der p 1, was developed as an edible vaccine. The C-terminal KDEL-tagged Der p 1 allergen specifically accumulated in seed endosperm tissue under the control of the endosperm-specific GluB1 promoter. Der p 1 reached a maximum concentration of 58 μg/grain and was deposited in the endoplasmic reticulum (ER)-derived protein body I (PB-I). Plant-derived Der p 1 was posttranslationally modified with high-mannose-type glycan structures. Glycosylated Der p 1 displayed reduced IgE binding capacity in comparison with its unglycosylated counterpart in vitro. Our results indicate that transgenic Der p 1 rice seeds are a safe, potential oral delivery vaccine for the treatment of HDM allergy. |
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Keywords: | House dust mite Oligomannose-type glycan Der p 1 allergen Transgenic rice Oryza sativa Endosperm tissue Edible vaccine |
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