Wnt5a-mediated non-canonical Wnt signalling regulates human endothelial cell proliferation and migration |
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Authors: | Cheng Ching-wen Yeh Ju-ching Fan Tai-Ping Smith Stephen K Charnock-Jones D Stephen |
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Institution: | a Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK b Department of Obstetrics and Gynaecology, The Rosie Hospital, Robinson Way, Cambridge CB2 2SW, UK c Department of Pharmacology, University of Cambridge, Cambridge CB2 1PD, UK |
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Abstract: | Cell to cell interaction is one of the key processes effecting angiogenesis and endothelial cell function. Wnt signalling is mediated through cell-cell interaction and is involved in many developmental processes and cellular functions. In this study, we investigated the possible function of Wnt5a and the non-canonical Wnt pathway in human endothelial cells. We found that Wnt5a-mediated non-canonical Wnt signalling regulated endothelial cell proliferation. Blocking this pathway using antibody, siRNA or a down-stream inhibitor led to suppression of endothelial cell proliferation, migration, and monolayer wound closure. We also found that the mRNA level of Wnt5a is up-regulated when endothelial cells are treated with a cocktail of inflammatory cytokines. Our findings suggest non-canonical Wnt signalling plays a role in regulating endothelial cell growth and possibly in angiogenesis. |
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Keywords: | Wnt Non-canonical Endothelial |
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