Ca and cell fusion during sexual development in liquid cultures of Dictyostelium discoideum |
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Authors: | Abdul H. Chagla Keith E. Lewis Danton H. O'Day |
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Abstract: | Cell fusion resulting in zygote giant cell formation is the first observable event of sexual development in D. discoideum. The results reported here show that this process is Ca2+-dependent and that by increasing the level of Ca2+ in the medium the number of cell fusions can be increased 57-fold over control cultures. The data also suggest that Ca2+ has both an early and late function in the development of zygotes and these functions are mediated at the cell surface. These results plus the availability of a liquid culture for generating large volumes of cells make sexual development in D. discoideum an excellent system for the analysis of membrane fusion in eukaryotes. |
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