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Effect of Copper on Levels of Collagen and Alkaline Phosphatase Activity from Chondrocytes in Newborn Piglets In Vitro
Authors:Xue Yuan  Jianguo Wang  Xiaoyan Zhu  Zhigang Zhang  Yongxing Ai  Guoquan Sun  Zhe Wang  Guowen Liu
Affiliation:College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, China.
Abstract:The effects of different concentrations of copper on collagen content and alkaline phosphatase (AKP) activity from chondrocytes in newborn piglets were measured. Chondrocytes were cultured in media containing 15% fetal calf serum supplemented with 0, 15.6, 31.2, and 62.5?μmol/L copper in a 12-well culture plate. Collagen content and AKP activity from the chondrocyte extracellular matrix increased significantly in the culture media with 15.6, 31.2, and 62.5?μmol/L copper and was the highest at 31.2?μmol/L copper (P?
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