Differential gene expression of wheat progeny with contrasting levels of transpiration efficiency |
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Authors: | Xue Gang-Ping McIntyre C Lynne Chapman Scott Bower Neil I Way Heather Reverter Antonio Clarke Bryan Shorter Ray |
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Institution: | (1) CSIRO Plant Industry, 306 Carmody Road, St Lucia, Qld, 4067, Australia;(2) CSIRO Livestock Industry, 306 Carmody Road, St Lucia, Qld, 4067, Australia;(3) CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia |
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Abstract: | High water use efficiency or transpiration efficiency (TE) in wheat is a desirable physiological trait for increasing grain
yield under water-limited environments. The identification of genes associated with this trait would facilitate the selection
for genotypes with higher TE using molecular markers. We performed an expression profiling (microarray) analysis of approximately
16,000 unique wheat ESTs to identify genes that were differentially expressed between wheat progeny lines with contrasting
TE levels from a cross between Quarrion (high TE) and Genaro 81 (low TE). We also conducted a second microarray analysis to
identify genes responsive to drought stress in wheat leaves. Ninety-three genes that were differentially expressed between
high and low TE progeny lines were identified. One fifth of these genes were markedly responsive to drought stress. Several
potential growth-related regulatory genes, which were down-regulated by drought, were expressed at a higher level in the high
TE lines than the low TE lines and are potentially associated with a biomass production component of the Quarrion-derived
high TE trait. Eighteen of the TE differentially expressed genes were further analysed using quantitative RT-PCR on a separate
set of plant samples from those used for microarray analysis. The expression levels of 11 of the 18 genes were positively
correlated with the high TE trait, measured as carbon isotope discrimination (Δ13C). These data indicate that some of these TE differentially expressed genes are candidates for investigating processes that
underlie the high TE trait or for use as expression quantitative trait loci (eQTLs) for TE.
Electronic Supplementary Material Supplementary material is available for this article at |
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Keywords: | Biomass production Drought Gene expression Microarray Transpiration efficiency Wheat |
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