The Conversion of 3-Indolylmethylglucosinolate to 3-Indolylacetonitrile by Myrosinase, and its Relevance to the Clubroot Disease of the Cruciferae |
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Authors: | SEARLE LINDA M; CHAMBERLAIN KEITH; RAUSCH THOMAS; BUTCHER DENNIS N |
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Abstract: | Methylene-14C]-3-indolylmethylglucosinolate (14C-IMG) was convertedin vitro to methylene-14Cl-3-indolylacetonitrile (14C-IAN)by myrosinase over a pH range of 4.0-6.0 and this conversionwas enhanced by ferrous ions. Other products of the reactionincluded 3-indolylmethanol, 3, 3'-diindolylmethane and ascorbigenA. Trace amounts of 14C-IAN were produced non-enzymically from14C-IMG in the presence of ferrous ion over a similar pH range.Furthermore, swede tissues (Brassica napus cv. Danestone) infectedwith Plasmodiophora brassicae Woron. could convert 14C-IMG to14C-IAN. These results were consistent with the hypothesis thatthe overgrowth symptoms of the clubroot disease are caused bythe conversion of IMG to the auxin precursor IAN. |
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