Detection of the Leghemoglobin Gene on Two Chromosomes of Phaseolus vulgaris by in situ PCR Linked-Fluorescent in situ Hybridization (FISH) |
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| Authors: | Uchiumi, Toshiki Kuwashiro, Ryoko Miyamoto, Junko Abe, Mikiko Higashi, Shiro |
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| Affiliation: | 1 Department of Chemistry and BioScience, Faculty of Science, Kagoshima University Kagoshima, 890-0065 Japan
2 Department of Earth and Environmental Sciences, Faculty of Science, Kagoshima University Kagoshima, 890-0065 Japan |
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| Abstract: | The leghemoglobin (Lb) gene on the metaphase chromosomes ofPhaseolus vulgaris was amplified by in situ PCR. The amplifiedLb gene could be detected on two chromosomes by fluorescentin situ hybridization (FISH) using the short Lb gene probe. (Received January 9, 1998; Accepted April 30, 1998) |
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