Kainate activated single channel cnrrents as revealed by domoic acid |
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| Authors: | M Sciancalepore Z Galdzicki X Zheng O Moran |
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| Institution: | (1) Scuola Internazionale Superiore di Studi Avanzati, Strada Costiera 11, 1-34014 Trieste, Italy;(2) Department of Biophysics, Academy of Medicine, ul. Chalubinskiego 10, PL-50 368 Wroclaw, Poland |
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| Abstract: | We have studied the properties of kainic acid receptor-activated channels using domoic acid as an agonist. Similarities of the electrophysiological, pharmacological and noise properties of domoic acid and kainic acid-evoked currents confirm that domoate is a potent and specific agonist of the kainate receptor. Single-channel properties of domoic acid-evoked currents were directly determined from outside-out membrane patches for the first time, and results were compared with those obtained by fluctuation analysis of macroscopic currents. Small conductance cationic-selective channels of  4 pS and a mean open time of 2 to 3 ms were detected using both methods.
Offprint requests to: O. Moran |
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| Keywords: | Domoic acid Kainic acid Excitatory aminoacids Patch-clamp |
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