Actinomycin D in low concentrations binds uniformly to human chromosomes |
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Authors: | A R Brothman T J Lindell |
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Institution: | Committee on Genetics and Department of Pharmacology, Arizona Health Sciences Center, Tucson, AR 85724, USA |
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Abstract: | The binding of actinomycin D (actD) to fixed human metaphase chromosomes was studied by using autoradiography with 3H]actD and indirect immunofluorescence with a specific anti-actD antibody. At concentrations of 0.01 and 0.1 micrograms/ml there was a uniform distribution of drug along the chromosomes as observed by both methods. This is the first study to date characterizing actD binding at such low concentrations to human chromosomes. Since actD intercalates into the DNA helix with GC specificity, our observations indicate that detectable differences in base composition along the lengths of human chromosomes are minimal. |
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Keywords: | To whom offprint requests should be sent Please request reprint numöber RP086 Current address: Millipore Corporation 80 Ashby Road Bedford MA 01730 USA |
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