Somatic embryogenesis and plant regeneration in wild cotton (Gossypium klotzschianum) |
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Authors: | Yuqiang Sun Xianlong Zhang Shuangxia Jin Shaoguang Liang Yichun Nie |
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Affiliation: | (1) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University Wuhan, 430070 Hubei, P.R. China |
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Abstract: | A simple and efficient method for high frequency somatic embryogenesis and plant regeneration from hypocotyl-derived cultures and suspension cultures of Gossypium klotzschianum Anderss, a wild, diploid species of cotton is described here. Embryogenic cultures were induced from hypocotyl sections on MSB medium with 0.9 M 2,4-D and 2.32 M kinetin. MSB medium containing 0.045 M 2,4-D, 0.93 M kinetin, 2.46 M IBA promoted embryogenic culture proliferation and embryo development. Suspension cultures with 0.23 M 2,4-D and 0.93 M kinetin also produced many embryos. Somatic embryos cultured on MSB medium with PGRs produced secondary embryos, and embryos developed into normal plantlets on PGR-free MSB medium. Regenerated plantlets were transferred onto the quarter-strength MSB medium with 0.5% active charcoal to avoid recallusing. Hypocotyls were better than cotyledons for culture induction and plant regeneration. 2,4-D and kinetin were essential for culture induction and maintenance. |
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Keywords: | Gossypium klotzschianum Anderss plant regeneration somatic embryogenesis |
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