Occurrence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis among captive mammals in the Bangladesh National Zoo |
| |
| Affiliation: | 1. Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh;2. College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China;3. Department of Microbiology and Public Health, Patuakhali Science and Technology University, Barishal 8210, Bangladesh;4. Bangladesh National Zoo, Mirpur, Dhaka 1216, Bangladesh;1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China;2. The Wild Animals Rescue Center of Henan Province, Zhengzhou 450044, China;3. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;1. École Nationale Supérieure Vétérinaire, Rue Issaad Abbes, El Alia, Alger, Algeria;2. Division of Foodborne, Waterborne and Environmental Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30329, USA;3. Department of Biology, Université Ziane Achor, Djelfa, Algeria;4. Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China;1. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou, Gansu 730046, China;2. College of Animal Science, Tarim University, Alar, Xinjiang 843300, China;3. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, China |
| |
| Abstract: | Cryptosporidium and Giardia are protozoan parasites capable of causing gastrointestinal illness in humans and animals. The purpose of this research was to determine the occurrence, genetic characteristics, and zoonotic potential of Cryptosporidium spp. and Giardia duodenalis in captive mammals at the Bangladesh National Zoo. A total of 200 fresh fecal samples from 32 mammalian species were collected and examined for Cryptosporidium spp. using nested polymerase chain reaction (PCR) targeting the small subunit (SSU) rRNA gene and G. duodenalis targeting the β-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) genes. The overall infection rates of Cryptosporidium and G. duodenalis among captive mammals in the zoo were 3.5% (7/200) and 5.5% (11/200), respectively. Five species/genotypes of Cryptosporidium (C. hominis, C. andersoni, C. muris, C. felis, and Cryptosporidium deer genotype) were identified. C. hominis was subtyped as IbA12G3 by sequence analysis of the glycoprotein 60 (gp60) gene. Multilocus genotyping of G. duodenalis revealed assemblages A, B, and D. Mixed infections of assemblages B and D and A and B were found in an Asiatic jackal and a Nilgiri langur, respectively. To our knowledge, this is the first report on the occurrence and genetic identity of the two parasites among zoo animals in Bangladesh. The results suggest that zoonotic Cryptosporidium spp. and G. duodenalis are maintained in and transmitted between captive mammals. Therefore, washing, cleaning, and disinfection measures should be implemented to reduce the spread of Cryptosporidium and G. duodenalis infections. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
