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Stepwise genetic engineering of Pseudomonas putida enables robust heterologous production of prodigiosin and glidobactin A
Affiliation:1. Chemical Engineering, School for Engineering of Matter, Transport, and Energy, Arizona State University, AZ 85287, ECG 301, 501 E. Tyler Mall, Arizona, 85287, United States;2. School of Life Sciences, Arizona State University, 427 E. Tyler Mall, Tempe, AZ, 85287, United States;1. Department of Microbial Natural Products, Helmholtz Institute for Pharmaceutical Research Saarland, Helmholtz Centre for Infection Research and Department of Pharmaceutical Biotechnology, Saarland University, 66123 Saarbrücken, Germany;2. Shandong University-Helmholtz Joint Institute of Biotechnology, Qingdao, P.R. China;3. Munich Center for integrated Protein Science (CiPSM) and Chair of Biological Chemistry, Technical University of Munich, 85354 Freising (Weihenstephan), Germany;4. BASF SE, 67056 Ludwigshafen am Rhein, Germany
Abstract:
Keywords:Heterologous expression  Genome editing  Polyketide  Non-ribosomal peptide
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