Molecular characterization of Oxyspirura mansoni and Philophthalmus gralli collected from the eyes of domestic chickens in Bangladesh |
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| Affiliation: | 1. Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Avenida Universidad 3000, Ciudad Universitaria, 04510 Mexico, City, Mexico;2. Centro de Investigación en Alimentación y Desarrollo, Sánchez Taboada Carret. al Varadero Nacional, km 6.6, Col. Las Playitas, Guaymas, Sector Varadero, Las Playitas, 85480 Heroica Guaymas, Sonora, Mexico;3. Laboratorio de Mamíferos Marinos, Centro para el Estudio de Sistemas Marinos (CESIMAR – CCT CONICET – CENPAT), Blvd. Brown 2915, 9120, Puerto Madryn, Chubut, Argentina;4. Universidad Nacional de la Patagonia San Juan Bosco UNPSJB, Sede Puerto Madryn, Blvd. Brown 3000, 9120, Puerto Madryn, Chubut, Argentina;5. Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, 37005 České Budějovice, Czech Republic;1. Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, 62100 Cuernavaca, Morelos, Mexico;2. Instituto de Biotecnología, Universidad Nacional Autónoma de México, 62210 Cuernavaca, Morelos, Mexico |
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| Abstract: | A variety of helminths have been found in domestic chickens in Bangladesh, but little is known about their gene sequences. Here, parasitic nematodes and trematodes were collected from the eyes of domestic chickens and analyzed for their morphological and morphometric characteristics, and characterized molecularly. The helminths were identified as Oxyspirura mansoni and Philophthalmus gralli. The ITS1 and ITS2 sequences of O. mansoni were 532 bp and 306 bp in length, respectively, and showed low identity (50.7–62.7%) with those of O. petrowi and O. conjunctivalis. Furthermore, the O. mansoni CO1 sequences (393 bp) showed five haplotypes (97.5–99.5% similarity) that formed a monophyletic clade. With respect to P. gralli, the ITS1 (452 bp) and ITS2 (736 bp) sequences showed 100% similarity with the reference sequences in GenBank. Both the ND1 and CO1 phylograms showed that P. gralli from Bangladesh, Costa Rica and Peru form a monophyletic clade, distinct from the clades of P. lucipetus and P. lacrymosus. Our data show that, Philophthalmus gralli isolates from Bangladesh, Costa Rica and Peru are genetically close to each other. |
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