Highly purified mitochondria from rat brain prepared by phase partition |
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Authors: | M.J. López-Pérez G. París C. Larsson |
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Affiliation: | 1. Department of Biochemistry, University of Lund, P.O. Box 740, S220 07 Lund Sweden;2. Department of Biochemistry, Faculty of Pharmacy, Universidad Complutense, Madrid 3 Spain |
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Abstract: | Mitochondria and synaptosomes from adult rat forebrain can easily be separated by counter-current distribution in an aqueous two phase system composed of Dextran T500 and poly(ethylene glycol) 4000. Both particles may also be separated by a batch procedure in which the same phase system is used. Electron micrographs and enzymatic activities show a high purity of the mitochondria obtained from the dextran-rich lower phase. Electron micrographs and enzymatic activities also show that intact synaptosomes can be obtained from the poly (ethylene glycol)-rich upper phase.The mitochondria purified by this method show good ADP/O ratios, respiratory control ratios, and state 3 rates. Synaptosomes showed a state 2-state 3 transition with no recuperation to state 4. |
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Keywords: | Mitochondria purification Phase partition (Rat brain) |
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