Transfer of the polyene antibiotic amphotericin B between single-walled vesicles of dipalmitoylphosphatidylcholine and egg-yolk phosphatidylcholine |
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| Authors: | Jacques Bolard Aline Vertut-Croquin Barbara E Cybulska Claude M Gary-Bobo |
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| Institution: | 1. Département de Recherches Physiques (LA CNRS 71), Université Pierre et Marie Curie, 4, Place Jussieu, 75230 Paris Cedex 05 France;2. Department of Pharmaceutical Technology and Biochemistry, Technical University, Gdansk Poland;3. Service de Biophysique, Département de Biologie, C.E.N. Saclay, 91191 Gif-sur-Yvette Cedex France |
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| Abstract: | Amphotericin B transfer between single-walled vesicles of dipalmitoylphosphatidylcholine (DPPC) and of egg phosphatidylcholine, both containing 10 mol% cholesterol, has been studied concurrently by circular dichroism spectroscopy and permeability measurements. At 22°C amphotericin B is rapidly transferred from DPPC to DPPC vesicles as well as from egg phosphatidylcholine to egg phosphatidylcholine vesicles. On the other hand, although amphotericin B is rapidly transferred from egg phosphatidylcholine to DPPC vesicles, it is not transferred from DPPC to egg phosphatidylcholine vesicles. At 48°C, above the transition temperature of DPPC, transfer occurs rapidly both ways. These results are interpreted in terms of difference of association constant of amphotericin B with vesicle membranes in the gel and liquid-crystalline state. |
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| Keywords: | Phosphatidylcholine vesicle Amphotericin B transfer Dipalmitoylphosphatidylcholine vesicle Circular dichroism DPPC dipalmitoylphosphatidylcholine DMSO dimethyl sulphoxide |
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